New Book-Series Chapter in Methods in Enzymology

18.09.2024

Rieske oxygenase enzymes play a key role in breaking down toxic chemicals into less harmful substances, making them essential for cleaning up contaminated environments. These enzymes are known for their ability to catalyze oxygenation reactions, however, a lesser-known side reaction, which produces reactive oxygen species (ROS), may be just as important in understanding how these enzymes function.

This recent book-series chapter summarizes methods to measure both productive oxygenation and unproductive ROS formation reactions, providing valuable insights into how these enzymes work and evolve, especially as microorganisms adapt to new environmental conditions. By understanding these reactions better, scientists aim to improve strategies for tackling environmental pollution at the molecular level.